Fig. 3. Meiotic defects in CTCF-depleted oocytes. Eggs having undergone GVBD by 2 hours of culture were processed for diakinesis spreads. Eggs that have undergone PBE by 16 hours of culture were processed for M2 spreads. Line 1 is shown. Seven percent (3/42) of Tg eggs that have undergone PBE contained roughly 20 bivalents. n=number of oocytes. Scale bar: 0.1 µm.