Fig. 5. Maternal pronuclear transfer and Ctcf mRNA-injection experiments. (A) Maternal pronuclear transfer experiments. Maternal pronuclei (mPNs) were reconstructed with one-cell embryos and cultured until 72 hours post-hCG. The average cell number per transfer-type at 72 hours post-hCG is indicated. Ntg and Tg (Line 1) indicate mPNs or embryos derived from Ntg and Tg littermates, respectively. Bars labeled A are significantly different from bars labeled B (overall P<0.0001). n=number of embryos. (B) Immunofluorescence images of CTCF-stained embryos after cytoplasmic mRNA microinjection. One-cell embryos from Line 1 were microinjected with CTCF or control GFP mRNA, and cultured until 72 hours post-hCG. One confocal section of an embryo is shown per panel, with red indicating CTCF-stained nuclei. Scale bar: 40 µm. (C) Embryo development after cytoplasmic mRNA microinjection. The average cell number per microinjection-type at 72 hours post-hCG is indicated. Bars labeled A are significantly different from bars labeled B (overall P<0.0001). n=number of embryos.