Fig. 3. Dap160 regulates embryonic neuroblast cortical polarity. (A-E) Wild type metaphase embryonic stage 15 neuroblasts stained for the indicated proteins (top labels) have apical crescents of aPKC, Bazooka, Par-6 (100%, n=23; 100%, n=34; and 96%, n=23, respectively) and basal crescents of Miranda (93%, n=43) and Numb (100%, n=21) (white arrowheads). (F-J) dap160 metaphase embryonic stage 15 neuroblasts. (F) aPKC is mostly ectopic cortical aPKC (71%, n=41, red arrow) or cytoplasmic (not shown). (G) Par-6 is ectopic cortical (50%, n=12, red arrow) or normal (not shown). (H) Baz is cytoplasmic (21.8%, n=96; H), ectopic cortical or normal (not shown). (I) Miranda is ectopic cortical (red arrow, 36%, n=55) or normal (not shown). (J) Numb is ectopic cortical (red arrow, 38%, n=18) or normal (not shown). Proteins are delocalized and may also be at lower levels than in wild-type neuroblasts. (K-M) dynamin mutant mitotic larval neuroblasts (shibire^ts/shibire^ts at restrictive temperature; see Materials and methods) have aPKC apical crescents (K) and basal crescents of Miranda (L) and Numb (M). (N) Quantification of the neuroblast cortical polarity phenotypes. Number of neuroblasts scored is shown as a number at the bottom of each bar. Scale bar: 5 µm.