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Figure 3


Fig. 3. Early Cre-induced recombination of Notch1 and Notch2 alleles in the pancreatic epithelium. (A,B) Detection of early {alpha} cells in the dorsal bud by immunostaining for glucagon at E12.5. (A) Microdissection of pancreatic epithelial cells. (B) Note that glucagon+ cells (dark) are not dissected. (C,D) Schematic maps of the floxed Notch1 (C) and Notch2 (D) locus before and after Cre-induced recombination. The position and polarity of the primers used for amplification are represented by P1, P2 and P3 for Notch1, and P4, P5, P6 and P7 for Notch2 (red arrowheads). DNA was isolated from pancreatic epithelial cells of two Notch1/2KO embryos (lane 1 and lane 2). As a control, DNA from heterozygous Notch1/2+/f mice was used (lane 3).