Fig. 5. Hh maintains Odd expression near the En cells. (A,A') Stage 11 hh^AC embryo stained for En (green), Odd (red) and Slp (blue). Odd is still present, whereas En disappears. (B,B') Stage 12 hh^AC embryo stained for En (green), Odd (red) and cadherin (blue). Some En-expressing cells remain, but no Odd cells are in the epidermis. Some Odd staining is visible in the nervous system. (C,C') Stage 12 ptc^IN embryo stained for En (green), Odd (red) and cadherin (blue). The Odd domain is wider than in the wild type (compare with Fig. 4H). (D,D') Stage 13 En-gal4 UASHh^WT embryo stained for En (green), Odd (red) and cadherin (blue). Odd is present in a four- or five-cell wide stripe. (E-E'') Detail of a stage 11 wild-type embryo (E) stained for Ptc (green), Cadherin (red) and Odd (blue). Both Ptc (E') and Odd (E'') domains are several cells wide. (F-F'') Detail of a stage 13 wild-type embryo (F) stained for Ptc (green), Cadherin (red) and Odd (blue). Both the Ptc (F') and the Odd (F'') domains are a single cell wide. (G-G'') Stage 13 ptc^IN embryo stained for En (green), aPKC (red) and cadherin (blue). Crb is enriched in the widened grooves. En face (G') and z-section (G'') detail from G. The groove on the left has ectopic En posteriorly, whereas the groove on the right does not. For 3D rotation movie, see Movie 4 in the supplementary material.