Fig. 6. Hh cannot induce grooves in dedifferentiated En cells. (A) Stage 13 prd-Gal4 UAS-Yan^act embryo stained for Yan (green), Cadherin (red) and Odd (blue; A'). Yan-expressing cells near the tracheal invagination move posteriorly between the two Odd populations. (B-B'') En-Gal4 UAS-Yan^act embryo stained for Ptc (green; B'), cadherin (red) and Odd (blue; B''). Ptc is expressed between the separated Odd populations. (C-C'') En-Gal4 UAS-Yan^act embryo stained for Yan (green), Cadherin (red, C') and aPKC (blue, C'') showing that the Yan-expressing cells do not accumulate aPKC and do not become rectangular. 3D visualization reveals an interruption in the groove (data not shown).