Fig. 3. Bioluminescence imaging demonstrates that cell grafts survive, proliferate and differentiate in vivo. (A) IVIS image of control injury showing no bioluminescence after luciferin injection. (B-E) Time course after L2G85 cell transplantation shows an initial increase of luciferase expression in the injury site, which slowly decreases over time. (F) GFP antibody staining of the injury site at post-surgical day 7 reveals immunopositive osteoblasts and osteocytes. Scale bar: 200 µm in F.