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Figure 1


Fig. 1. Dorsal spinal cord neurons express RhoA, ROCK and PRK family members. (A) RT-PCR amplification indicates that RhoA, RhoB and RhoC, as well as ROCKI, ROCKII, PRK1 and PRK2 are expressed by SCNs. (B) Western blot analysis of SCN lysate using RhoA, ROCKII and PRK2 antibodies. Immunofluorescent localization of RhoA (C,D), ROCKII (G,H) and PRK2 (K,L). In E,F,I,J,M,N. DCC is labeled green and nuclei blue; RhoA (E,F), ROCKII (I,J) and PRK2 (M,N) are labeled in red. Scale bar: 20 µm for C,E,G,I,K,M; 10 µm for D,F,H,J,L,N.