Fig. 4. Increased RhoA activity reduces SCN axon outgrowth evoked by netrin 1. (A-I) SCN explants expressing RFP, wt-RhoA or ca-RhoA were cultured for 14 hours in the presence of 200 ng/ml netrin 1. (B) Wt-RhoA reduced total outgrowth by 53% (n=21, P<0.001) and (C) ca-RhoA reduced it by 79% (n=23, P<0.001) compared with RFP infected explants (A). C3-07 increased RFP infected explant outgrowth by 32% (n=23, P<0.001) (D), wt-RhoA by 72% (n=20, P<0.001) (E) and ca-RhoA by 122% (n=20, P<0.001) (F). Y27632 increased RFP infected explant outgrowth by 53% (n=19, P<0.001) (G), wt-RhoA by 155% (n=20, P<0.001) (H) and ca-RhoA by 288% (n=22, P<0.001) (I). (J)^**P<0.001 compared with control RFP explants. ##P<0.001 compared with wild type or CA explants. Tukey post-hoc tests of means. Error bars indicate s.e.m. Scale bar: 100 µm.