Fig. 5. Fgf8 electroporation expands the posterior vLGN and EML. Electroporated brains were collected at E15.5, sectioned coronally and examined by two-color in situ hybridization. Anterior is towards the bottom and posterior is towards the top according to the longitudinal axis (A,E). A closer view of vLGN and the EML region is shown (B-D,F-H). Nkx2.2 (blue) marks the posterior vLGN and EML, while Arx (brown) marks the anterior vLGN and EML (blue and yellow arrows, respectively, B). Gad67 (blue) labels all of PTh but not the small medial population in vLGN, which is stained by Pitx2 (brown) (green arrow, F). The posterior vLGN is expanded posteriorly and position of posterior EML is abnormal in Fgf8-overexpressing brain (arrows in C,G) but without alteration of anterior structures labeled by Arx (brown staining, B,C) or the small medial population of vLGN labeled by Pitx2 (brown staining, F,G). Inhibition of Fgf8 decreased the size of the posterior vLGN (D,H, arrows), but no change was observed in the anterior vLGN (D) or Pitx2+ population (H). Scale bar in E: 500 µm for A,E; 200 µm for B-D,F-H.