Fig. 2. GAL4-GH146-positive projection neurons are subsets of the antennal lobe neuron lineages. (A-C) Illustrations of antennal lobe neuron lineages and cellular composition. (A) GAL4-GH146-positive projection pathways of PNs. Abbreviations: iACT, inner antennal-cerebral tract; mACT, middle ACT; LH, lateral horn; MB, mushroom body. (B,C) Proportion of GAL4-GH146- and Acj6-positive PNs in the AL adPN, lAL and vPN lineages. (D-F) Composite confocal images of three AL lineages (D1'',D2'',E1'',E2'',F1'',F2'') that generate GAL4-GH146-positive PNs (D1',D2',E1',E2',F1',F2'). The Nb clones are generated at early larval stage and labeled by dual-expression-control MARCM. D1, E1 and F1 show the projection patterns and are merged from D1',D1'', E1',E1'' and F1',F1'', respectively. D2, E2 and F2 are the single confocal images of cell bodies magnified from D1, E1 and F1 and are merged from D2',D2'', E2',E2'' and F2',F2'', respectively. (G-I) The composite confocal images of the location and composition of Acj6-positive neurons in the adPN, lAL and vPN lineages. The MARCM Nb clones are labeled with tubP-GAL4 (white) and counterstained with Acj6 and nc82 (blue). The broken white lines outline the AL. (J-J'') Projected confocal images of the PNs co-labeled by GAL4-GH146 (J') and LG-GH146 (J''). J shows the merged images from J' and J''. The GAL4 and LG drivers differ only in the intensity of some expression. Scale bars: 20 µm.