Fig. 2. roe loss-of-function affects the expression of N-signaling targets. Panels show third instar mosaic imaginal discs bearing rn¹⁶ clones, marked by absence of arm-lacZ (red in A,E,F) or Ubi-GFP (red in B-D) and outlined in white in right panels. -Elav is green in B-E. (A,A') -Ato (blue) is detected as a continuous band at the MF and resolves into individual cells (R8) posterior to it. -Ato is reduced in single cells inside the mutant clone (arrows) when compared with surrounding wild-type tissue (arrowheads). (B,B') The Ato reporter line ato5'F9.3-Z (blue), which is repressed by N-signaling during R8 lateral inhibition, is not detected inside mutant tissue. (C,C') The E(spl)mβ1.5-Z reporter line (blue), which is regulated by N signaling, shows higher expression levels inside mutant clones when compared with surrounding wild-type cells. (D,D') The E(spl)m1.1-Z reporter (blue), which is activated by N signaling, is expressed in isolated cells posterior to the MF. Inside mutant tissue, more positive cells are observed when compared with surrounding wild-type tissue. (E-F') Neither N (E,E', blue and monochrome, detected by -N_intra) nor Dl expression levels (F,F', turquoise and monochrome) are affected inside mutant tissue.