Fig. 1. JIL-1 and histone H3S10ph antibody labeling of Drosophila salivary gland nuclei smush preparations. JIL-1 (green) and histone H3S10 phosphorylation (H3S10ph, red) co-localize on chromosomes from both female (top row) and male (middle row) wild-type (wt) nuclei. The middle row illustrates the characteristic upregulation of JIL-1 and H3S10ph labeling on the male X chromosome (X). Labeling of both JIL-1 and H3S10ph is absent in JIL-1^z2/JIL-1^z2 (z2) null mutant nuclei (lower row). The H3S10ph antibody used was from Cell Signaling.