Fig. 6. Immunoblot analysis of H3S10ph in mutants of the 55 kd regulatory subunit of Protein phosphatase 2A. (A) H3S10ph antibody labeling of protein extracts from wild-type (wt) and tws⁰²⁴¹⁴/tws⁰²⁴¹⁴ (tws⁰²⁴¹⁴) whole third instar Drosophila larvae. (B) H3S10ph antibody labeling of protein extracts from wild-type and tws⁰²⁴¹⁴/tws⁰²⁴¹⁴ salivary glands. (C) H3S10ph antibody labeling of protein extracts from tws^P/tws^P (tws^P) and JIL-1^z2/JIL-1^z2 (z2) salivary glands with (HS) and without heat shock treatment. The H3S10ph antibody was from Epitomics (epi); labeling with histone H3 (H3) antibody was used as a loading control.