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Figure 1


Fig. 1. Manipulating the expression of B{alpha} and B{delta} in Xenopus embryos produces distinct phenotypes. (A) Xenopus embryos were injected with either control GFP mRNA, Flag-tagged mouse B{delta} mRNA (B{delta}), a morpholino control (MoC) or a specific morpholino against Xenopus B{alpha} (MoB{alpha}) or B{delta} (MoB{delta}) at the one-cell stage, and fixed when control embryos had reached either early gastrula or tailbud (stage 25). Representative embryos are shown, arrowheads indicate anterior. The anterior regions of embryos are magnified below. Head structures are lacking in B{delta}-injected embryos and MoB{alpha}-injected embryos. (B) Embryos were injected as in A with the indicated mRNAs and morpholinos. The effect of MoB{delta} or MoB{alpha} could be rescued by co-injection with the cognate mRNA (mouse B{delta} or B{alpha}). Percentages of embryos showing wild-type phenotype when control-injected embryos had reached stage 22 are given. Arrowheads indicate anterior.