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Figure 2


Fig. 2. Manipulating the expression of B{alpha} and B{delta} in Xenopus embryos has opposing effects on Activin/Nodal target gene expression. (A) In situ hybridisation of gastrula-stage embryos injected with either a morpholino control (MoC) or a specific morpholino against Xenopus B{alpha} (MoB{alpha}) or B{delta} (MoB{delta}), or with Flag-tagged mouse B{delta} mRNA (B{delta}) at the one-cell stage. The probes used were against gsc or Xbra. Staining was visualised with BM purple. There is increased staining for both Xbra and Gsc in B{delta} morphants, and decreased staining in B{alpha} morphants and embryos overexpressing B{delta}. (B) Analysis of gene expression by q-PCR. Total RNA was isolated from stage 10.5 embryos that had been injected at the one-cell stage with either morpholino control, or morpholinos against B{alpha} or B{delta}. Expression levels are normalised to ornithine decarboxylase (ODC).