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Figure 5


Fig. 5. Inhibition of PI3-kinase or MAP-kinase signaling abolishes the anterior PS or mesoderm induction, respectively. The {Delta}Nβ-cateninER cells were cultured with 4OHT and Noggin (Nog) in the presence or absence of U0126 (U) and LY294002 (LY) for 3 days, and then analyzed by qPCR (A), and stained with specific antibodies as indicated (B). v, vehicle control. Scale bar: 100 µm.