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Figure 1


Fig. 1. Genotyping and phenotyping SM22{alpha}-Cre;Bmpr1aflox/flox mouse embryos. (A) Genotyping of mouse embryos. Targeted deletion of exon 2 of Bmpr1a occurs only in mice expressing Cre and the floxed Bmpr1a gene. SM22{alpha}-Cre;Bmpr1aflox/+ (f/+) and flox/flox (f/f) represent mice heterozygous and homozygous for the floxed gene, respectively. Mice expressing either Cre or the floxed Bmpr1a gene represent the WT group. Any mutant (f/fR26R) or WT (WTR26R) mouse expressing Cre and the R26R gene, expresses β-galactosidase and can be used for lacZ staining. (B) Cre activity in SM22{alpha}-Cre;R26R;Bmpr1aflox/flox embryos. Whole-mount lacZ staining of SM22{alpha}-Cre;R26R;Bmpr1aflox/+ embryos showing blue staining (a) in the heart (asterisk) and dorsal aorta (arrow) at E9.25, and (b) in smaller vessels as well as somatic myotomes (arrows) at E10.5. (C) Phenotype of SM22{alpha}-Cre;R26R;Bmpr1aflox/flox (b,d,f) compared with WT (a,c,e) embryos. SM22{alpha}-Cre;R26R;Bmpr1aflox/flox mutants appear normal at E9.5 (b versus a) and relatively reduced in size at E10.5 (d versus c); at E11, areas of hemorrhage are noted in the heart (asterisk) and abdomen (asterisk), as well as near the mouth (arrow) and brain (arrowhead) (f versus e). (D) Percentage of total mice of SM22{alpha}-Cre;R26R;Bmpr1aflox/flox genotype. Numbers of genotyped mice of each age are depicted in parentheses.