Fig. 2. Time-lapse imaging of vsx1-GFP cells showing that V2a and V2b neurons are produced as siblings. (A,B) Time-lapse imaging of vsx1-GFP cells, followed by immunohistochemical localization of Chx10 (red, V2a) and Scl (green, V2b). (A) Lateral view. (B) Dorsal view. Images are montages of stacked optical sections. Green arrowheads indicate cells prior to division. Red arrowheads indicate paired cells after division. Movies for these analyses are available in the supplementary material. (C) Summary of time-lapse imaging.