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Figure 2


Fig. 2. Colonisation of the gut of E8.5+3 embryos transplanted with Ret+ ENS progenitors. (A) DiI labelling of NCCs at the level of somites 2-4 results in efficient colonisation of the gut by fluorescent cells. (B) E8.5+3 wild-type mouse embryos transplanted with Ret+ cells isolated from the intestine of E11.5 Rosa26bgeo embryos. At the end of the culture period, β-geo+ cells (arrow) are restricted to the gastrointestinal tract. (C,D) Examples of the two classes of embryos classified according to the extent of gut colonisation by grafted cells. st, stomach; mg, midgut; cae, caecum.