Fig. 2. phyl mediates G2-M transition by positively regulating stg mRNA expression. (A,B) stg mRNA expression pattern at the AWM of wild-type (A) and phyl²/phyl⁴ (B) wing disks at 2-4 hours APF. Arrows indicate the stg-positive SOPs. In phyl mutants, stg expression is significantly reduced in AWM SOPs (B). (C-F) Ectopic expression of stg rescues the defects in G2-M transition. (C) Percentage of cell division of thoracic SOPs at 22-24 hours (column 1 and 2) and 24-26 hours (column 3-4) APF. SOP division defect is strongly rescued when stg was added back transiently to phyl mutant pupae by heat-shock treatment (lanes 2 and 4). ^*P<0.0005, n=4-9 thoraces. The numbers in the parenthesis are the number of SOP scored. (D-F) Images of pupal thoraces stained with anti-Sens antibody (green). (D) Wild-type. (E) phyl²/phyl⁴; hs-Gal4/+ pupa with two pulses of heat-shock treatment. (F) phyl²/phyl⁴; hs-Gal4 UAS-stg/+ pupa with two pulses of heat-shock treatment.