Fig. 4. phyl downregulates Sc but not Da protein levels in SOPs. (A-E) Anti-Sc or anti-Da staining is shown in red, and SOPs were labeled by anti-Sens staining (green). (A-A'') Sc expression pattern in the wild-type pupal thorax at 12-13 hours APF. Sc levels are high in newly specified SOPs (arrowheads), but gradually diminishing in more mature SOPs (arrows). (B-B'') Thoracic region of Eq-Gal4/UAS-myc-sc wing disk. Although Myc-Sc (red) is clearly detected in most non-SOP disk cells, its protein levels are strongly downregulated in late-stage single SOPs (arrows). (C-C'') Sc protein is accumulated in the phyl² mutant SOPs (GFP-negative) at 14-15 hours APF. (D-E'') Da protein levels in AWM SOPs. Da protein (red) is expressed in both newly specified SOPs (arrowheads) at late third instar (D-D'') and in mature SOPs (arrows) at 2-4 hours APF (E-E'').