Fig. 7. Mutations in ac and sc rescue SOP division defect of phyl mutants. (A-F) Pupal thoraces stained with anti-Sens (green) and anti-Hnt (red) antibodies to label all SOP daughter cells. (A) Wild-type pupal thorax at 24-26 hours APF. All SOPs have divided into three-to five-cell clusters. (B) In female phyl²/phyl⁴ pupal thorax at 24-26 hours APF, 27% microchaetal SOPs have divided into two cells and almost no microchaetal SOP has divided into a three-to five-cell cluster. Arrowheads indicate SOP daughter cells of macrochaete (large bristle). Macrochaetal SOPs were not counted in this assay due to different division timing (Huang, 1991). (C) Female sc^10-1/+; phyl²/phyl⁴ pupal thorax. Arrows indicate SOPs divided into three-to four-cell clusters. (D) Male phyl²/phyl⁴ pupal thorax at 22-24 hours APF. 27.8% SOPs have divided. Arrowheads, SOP daughter cells of macrochaetes. (E,F) ac^cami/Y; phyl²/phyl⁴ (E) and sc^M6/Y; phyl²/phyl⁴ (F) pupal thoraces at 22-24 hours APF. Arrows indicate SOPs divided into three- or four-cell clusters. Arrowheads in E, SOP daughter cells of macrochaetes. (G) Percentage of SOP division in thoraces at 22-24 hours APF. ^**P<0.0005, ^*P<0.01, n=7-22 thoraces. The numbers in parentheses are the number of SOP scored. Mutant pupae with fewer than 10 SOPs were not scored.