Fig. 1. Bowl accumulates in the PE and is absent from the DP. Spatiotemporal expression pattern of Bowl (red), wg-lacZ (green) or odd-lacZ (green). Optical sections at the plane of the PE (A,C,E) and DP (B,D,F). (A,B) 48 hours AEL; (C,D) 72 hours AEL; (E,F) 96 hours AEL. wg-lacZ was detected in the DP in a wedge of ventral cells (arrow in B). wg was upregulated along the DV compartment boundary (arrows in D and F). Bowl accumulated broadly in the PE (A,C,E) and was absent from the DP (B,D,F), except for the lateral margins of the notum (arrowheads in D and F). (G,H) 120-144 hours AEL; Bowl accumulated in the anterior margin of the PE (asterisks in G and H) and the lateral margins of the notum (arrowheads in H). However, Bowl was downregulated in the medial and posterior regions of the PE. (I) Everting discs; Bowl accumulated in the ventro-anterior hinge (asterisk) and in the lateral margins of the notum (not shown in this image). (J) odd-lacZ was detected in a similar pattern at this stage (arrowheads indicate the lateral margins of the notum, asterisks the ventro-anterior hinge). (K) Adult wings; odd-lacZ was detected in ventro-proximal hinge nuclei. (L) A schematic diagram illustrating the regulatory interactions linking drm, lines and bowl.