Fig. 4. The DV, AP and PD patterning systems are elaborated in the absence of the PE. Analysis of Ubx>Lines discs with molecular markers for the PE and for the AP, DV and PD patterning systems. (A,E,G,I,K) Wild type; (B-D,F,H,J,L) Ubx>Lines; maximal intensity projections. Arrowheads in D,H,J,L indicate the plane of z-sections. (A) Wild type; Ubx (red) and Bowl (green) are expressed in the PE in nested domains. Ubx occupies a broad central domain. Bowl occupies a broader domain. (B-D) Ubx>Lines; expression of the peripodial markers Bowl (B) and Ubx (C) is absent; arrow in B indicates a leg where Bowl accumulation is not affected. (D) Lines was broadly nuclear. (E) Wild type; Vg (red) marks the pouch; Zfh2 (green) marks the hinge. (F) Ubx>Lines; Vg and Zfh2 were expressed in adjacent domains in both layers of the wing disc. The Zfh2-negative notum formed a tiny rudiment (arrow). (G) Wild type; Wg is restricted to the DP. (H) Ubx>Lines; Wg (red) was detected in the pouch (short arrow) and hinge (long arrows) in both layers of the disc epithelium but not in the notum. In wild type, (I) Dll and (K) Sal are induced in response to Wg and Dpp signaling, respectively. In Ubx>Lines, (J) Dll (red), Zfh2 (green) and (L) Sal were induced in both layers of these discs. Scale bar: 100 µm in B-D,F-L; 200 µm in A,E-K.