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Figure 1


Fig. 1. Pdgfr{alpha} is localised to the PrE layer of E4.0 blastocysts and blastocyst outgrowths. (A) Pdgfr{alpha} is co-expressed with Gata4 in the PrE of E4.0-4.5 blastocysts. (B-E) Co-expression of GFP in E4.0 and E4.5 Pdgfr{alpha}H2B-GFP/+ embryos with Pdgfr{alpha} protein (B), and with the PrE markers Gata6 (C), Gata4 (D) and Dab2 (E). (F) GFP is co-expressed with Gata4 in Pdgfr{alpha}H2B-GFP/+ blastocyst outgrowths after 72 hours of culture. Each row represents one embryo. All panels show single optical sections. bf, bright field; green, Pdgfr{alpha} (A) and GFP (B-F); red, Pdgfr{alpha} (B), Gata4 (A,D,F), Gata6 (C) and Dab2 (E); blue, Hoechst. Scale bar: 20 µm.