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Figure 2


Fig. 2. Expression of GFP in live Pdgfr{alpha}H2B-GFP/+ embryos during pre- and early postimplantation development. (A-C) Heterogeneous GFP distribution in a 16-cell morula (A), a 36-cell early blastocyst (B; arrowhead indicates GFP-expressing TE cell) and a 64-cell mid-blastocyst (C). (D) In an ~80-cell blastocyst, GFP-expressing cells are partially segregated to the prospective PrE layer. (E,F) In an ~100-cell blastocyst (E), GFP-expressing cells are restricted to the PrE layer and in a 120-cell blastocyst (F) they start to migrate along the mural TE (arrowheads). (G) At E5.5, GFP-positive cells occupy the visceral endoderm (arrowhead indicates remainder of GFP-positive parietal endoderm). The second column depicts 3D reconstructions of the z-stacks, other panels show single optical sections. bf, bright field; green, GFP; red, FM4-64. Scale bar: 20 µm.