Fig. 3. Localisation of Nanog and Gata6 in embryos from 8-cell to blastocyst stage. (A) Gata6 and GFP expression in a 16-cell Pdgfra^H2B-GFP/+ embryo. (B) Gata6 and Nanog are co-expressed in 8-cell embryos. (C) In a 32-cell morula, Nanog and Gata6 show broad, overlapping expression. (D) In a 33-cell blastocyst, Nanog and Gata6 colocalise in some, but not all cells. (E) In a 58-cell blastocyst, Gata6 expression is mutually exclusive from that of Nanog. Each row represents a single optical section of one embryo. bf, bright field; green, GFP; white, Nanog; red, Gata6; blue, Hoechst. Scale bar: 20 µm.