Fig. 10. Mutational evidence that the ECudA core region is the DNA-binding domain. His-tagged ECudA, either wild-type (WT) or mutated in the two positions indicated in Fig. 9A (LSS, RVISK) and described in the text, was analysed by band shift using the 4-fold multimerised 14-mer element as a probe. In the bar chart, the averaged results of four experiments are quantitated and presented with the s.d.