Fig. 6. Demonstration that CudA binds to the ECudA dyad element. CudA purified from Dictyostelium nuclear extracts using wild-type and mutant versions of the 4-fold multimerised 14-mer element, defined using ECudA, was analysed by western blotting. Non-specific binding was determined in a parallel purification using beads (no DNA). Results from three experiments are compiled in the bar chart. The amount of bound CudA is shown as amount bound, after subtracting non-specific binding and relative to the WT sequence with the indicated s.d. values.