Fig. 3. Mwh is an FH3-domain protein that localises more strongly proximally in cells. Adult (A-C) or pupal (E-G'') Drosophila wings. (A) mwh¹ adult wing between veins 3 and 4, showing trichomes with characteristic multiple wing hair phenotype. (B) Wing expressing CG13913-IR under control of ptc-GAL4 between veins 3 and 4 showing a multiple wing hair phenotype. (C) mwh¹ wing expressing EGFP-CG13913 from a P{w+, ActP-EGFP-CG13913} transgene showing rescue of the multiple wing hair phenotype. (D) Diagram of the CG13913 coding sequence, showing the extent of the Interpro GBD/FH3 (IPR014768) and FH3 (IPR010472) domains, and the point at which the inversion in mwh¹ breaks the coding sequence (367, arrow). (E-E'') mwh¹ clone in a 32-hour wing, labelled for Mwh (magenta), clonal marker (lacZ, green) and Fmi (blue). (F-F'') mwh¹ clone in a 32.5-hour wing, labelled for Mwh (green) and actin (Ph, magenta). Arrowheads indicate trichomes in which Mwh does not colocalise strongly with actin bundles. (G-G'') mwh¹ clone in 32.5-hour wing, labelled for Mwh (green) and -tubulin (magenta). Arrowheads indicate trichomes in which Mwh does not colocalise with -tubulin. Scale bars: 50 µm in A; 10 µm in G''.