Fig. 5. Mwh gel-mobility is modified by frtz activity and phosphorylation. Immunoblots using anti-Mwh on extracts from Drosophila pupal wings of the indicated genotypes, with w¹¹¹⁸ as wild-type control. Positions of molecular weight markers shown on left; arrow on right indicates Mwh signal; arrowhead points to non-specific band that is not altered in mutant extracts. Some extracts also show a weak higher-molecular-weight band which appears to be specific (asterisk), but which varies in intensity. Blots were reprobed for actin or tubulin as loading controls. (A) Extracts from 32-hour pupal wings at 25°C. (B) Extracts from 32-hour pupal wings at 25°C, either treated with the phosphatase inhibitor microcystin (control, w¹¹¹⁸) or with lambda phosphatase (P'ased). (C) Extracts from 28-hour pupal wings raised at 29°C or 64-hour pupal wings raised at 18°C (both equivalent to 32 hours at 25°C).