Fig. 2. Expression levels of Cluster II/III genes in single-cell cDNAs. Data from the probe sets that were expressed in Cluster II/III, but not in Cluster I, were used for clustering (n=117 probe sets; Welch's t-test, FDR<0.1, log fold-change cut-off >2.5). Each column indicates one cell, and each row indicates one probe set on the microarray. The expression levels are color-coded from red (high) to black (low). These probe sets were categorized into several groups based on their expression pattern along the clusters: probe sets that showed a typical expression pattern are grouped by color (blue, green or yellow). The in situ hybridization patterns of these genes are shown in Fig. 3. For the I^- II⁺ III⁺ IV^- genes (yellow), the log fold-changes from Cluster II to Cluster III cells are also indicated.