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Figure 3


Fig. 3. mRNA expression of Cluster II/III genes in the E14 mouse brain. In situ hybridization of all of the (A) yellow (I- II+ III+ IV-) and (B) green (I- II- III+ IV-) genes, and some of the (C) blue (I- II+/- III+ IV+) genes from Fig. 2. Magnified views of Ac,b,f are shown in Am,n,o, respectively; Bu,v show magnified views of Bh,k, respectively; Ci shows a magnified view of Cg. Signals for the I- II+ III+ IV- genes typically exhibited a two-band pattern in the SVZ and part of the VZ (arrows, Ac,d,h,i,j,k,m; see also Bj,r). For some genes, the band in the VZ was dominant (arrowhead, Aa,b,f,g,n,o). In both cases, the VZ signals were 20-40 µm from the apical surface (dotted line). GenePaint set IDs are indicated at the bottom right of some panels. Scale bars: 50 µm.