Fig. 2. Transition of hnRNP K from predominantly nuclear to mixed nuclear and cytoplasmic localization during neuronal development. (A-C) hnRNP K immunofluorescence (A), DAPI-fluorescence (B) and phase-contrast (C) images of embryonic muscle cell and neurons in dissociated cell culture. hnRNP K immunostaining colocalized with DAPI-stained nuclei (arrowheads, A,B); neurites and growth cones (gc) had no immunostaining (arrows in A,C). (D,E) hnRNP K immunoperoxidase staining of sections of juvenile frog retina (D) and spinal cord ventral horn (E). Retinal ganglion cells (RGC) exhibited more intense cytoplasmic than nuclear staining; motoneuron staining was both in the nucleus and cytoplasm (arrowhead, E). Arrow in E indicates interneuron with predominantly nuclear staining.