Fig. 7. Effects of hnRNP K knockdown on neurite outgrowth in culture. (A,A') Neuron from control MO culture with neurite. (B,B') Cells from bilaterally injected hnRNP K MO cells lacking neurites. Arrowheads in A and B indicate cells identified as neurons because they were positive for NF-M mRNA (by FISH). (C) The fraction (±s.d., n=4 cultures) of NF-M mRNA-positive cells that had neurites. In parentheses are the total number of NF-M mRNA-positive cells scored. Asterisks indicate significant variation relative to control MO and uninjected cultures (P<0.01, one-way ANOVA). (D-E') Cells in control cultures immunostained for NF-M. Whereas most cells that expressed NF-M had neurites (D), a few did not (E). Scale bars in A and D apply to A-B' and D-E', respectively.