Fig. 9. Polysome profiling of NF-M and peripherin mRNAs. Black bars indicate the distribution of NF-M or peripherin mRNA across fractions, represented as a percentage of the total of each respective mRNA in the gradient (right ordinate). The black line depicts A₂₆₀ values (left ordinate) across fractions. `M' indicates the position of the monosomal peak in the A₂₆₀ trace. (Top four panels) During normal development, NF-M (left) and peripherin (right) profiles shift from right to left, indicating mRNA moving from poorly translated to efficiently translated fractions. (Bottom two panels) Bilaterally injected hnRNP K MO1 embryos processed at stage 29/30. hnRNP K knockdown strongly inhibited the shift into heavier polysomal fractions for NF-M but not peripherin mRNA.