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Figure 3


Fig. 3. Activated PKA can compensate for reduced Gsk3β or E(Spl) activity to promote IZ formation. Zebrafish embryos analyzed at the 3- to 5-somite stage for neurog1 and pax2.1 and flat-mounted, anterior up. Bracket, MIZ; arrows indicate ectopic neurog1 expression. (A-D) Activated PKA (PKA*, injected as capped RNA at the one-cell stage) does not affect IZ formation (compare A with D, see also F) and blocks LiCl-induced neurog1 expression (arrows) across the MIZ (compare B with C). (E-H) Coinjection of PKA* RNA and her5GripNA (G) blocks the neurogenic effect of her5GripNA alone (E, arrows) and restores the MIZ. (H) Control.