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Figure 1


Fig. 1. Subepicardial and intramyocardial blood vessels represent venous and arterial lineages. (A-F) Immunofluorescent staining of cryosections for PECAM (A,D; red) and lacZ (B,E; green). Staining of E12.5 Efnb2-lacZ mice (A-C) reveals that intramyocardial (arrow), but not subepicardial (arrowhead) blood vessels express the arterial marker, ephrin B2. Similar analysis of E12.5 Ephb4-lacZ (D-F) mice demonstrates that subepicardial (arrowhead), but not intramyocardial, blood vessels (arrow) express the venous marker Ephb4. (G-L) Immunofluorescent staining of cryosections from Efnb2-lacZ (G-I) and Ephb4-lacZ (J-L) adult hearts shows that the spatial relationship between larger arteries and veins is conserved in the adult heart. Green, PECAM (G,J); red, lacZ (H,K). (C,F,I,L) Merges of fluorescent signals from A and B, D and E, G and H, and J and K, respectively.