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Figure 3


Fig. 3. Acinar cells in PMycKO embryos. Serial sections of E14.5 wild-type and PMycKO embryos stained for Myc (A,B, black, arrows) and amylase (C,D, brown, arrows). (E,F) Double-immunofluorescence staining of wild-type and PMycKO sections for amylase (green) and BrdU (red) at E15.5. Arrows indicate the BrdU-positive and arrowheads the BrdU-negative acinar cells. (G) BrdU+ nuclei were counted in amylase immunoreactive cells. Inserts in A and C represent a 4x enlargement and a 2x enlargement in E. Abbreviations: ac, acini; l, liver; p, pancreas. Scale bar: 50 µm.