Fig. 2. LH reduces gap junction permeability between mural granulosa cells. Determined by fluorescence redistribution after photobleaching of Alexa Fluor 488. (A) No LH. (B) LH applied 58 minutes before photobleaching. For A and B, a rectangular region was photobleached for 4.5 seconds, between the images indicated by the arrow. Each column shows images before and at various times (in seconds) after the end of the bleach. (C,D) Fluorescence intensity in the bleached region as a function of time, for the images shown in A and B. (E) Percent recovery of fluorescence intensity in the bleached region during the first minute after the bleach, as a function of LH treatment time. Bars show mean±s.e.m., and the numbers in parentheses indicate the number of follicles tested at each time point (0.5 hour=27-40 minutes, 1-2 hours=58-120 minutes).