Fig. 4. Immunofluorescence of Cx43 in antral follicles with or without a 1 hour exposure to LH. (A) Cumulus-oocyte region. Upper panels show scanning transmission differential interference contrast (DIC) images, middle panels show total Cx43 fluorescence, and lower panels show overlayed images. (B) Mural granulosa region, showing overlayed images as in A. The images are representative of eight follicles without LH, and seven with LH; both the cumulus and the mural granulosa regions were examined in each follicle.