Fig. 4. The spindle equatorial region is partially defective in the incenp mutant. (A,B) Immunolocalisation of Incenp and Aurora B localises to the equatorial region of meiotic metaphase I spindle in wild type and the incenp^QA26 mutant. (C) Immunolocalisation of Cyclin B to the equatorial region of meiotic metaphase I spindle in wild type and the incenp^QA26 mutant. (D,E) Examples of GFP signal intensity plots along the spindles from wild-type and incenp oocytes expressing GFP-tubulin, as marked with the red line in E. (F) The relative intensities of the spindle equatorial region (maximum intensity within the central 4 µm) over pole regions (average maximum intensity of 2 µm from each pole) are shown as the mean values (bars; 1.4 in wild type and 1.1 in incenp) and standard deviations (lines) for multiple wild-type and incenp mutant spindles. The difference is significant (P<0.015; n=15). Scale bars: 10 µm.