Fig. 5. The incenp mutation delays spindle microtubule assembly in female meiosis. (A) Time taken from nuclear envelope breakdown to the first appearance of spindle microtubules around the chromosomes in wild-type and incenp^QA26 mutant oocytes expressing GFP--tubulin. The mean values (bars) are shown with standard deviations (lines). The difference is significant (P<0.001; n8). (B) The length of metaphase I-arrested spindles in wild type and the incenp^QA26 mutant. (C) The relative amounts of -and -tubulins in ovaries from wild type and the incenp^QA26 mutant were examined by immunoblots. Blotted membranes are stained with MemCode for loading and transfer controls.