Fig. 6. Subito is required for stability of the spindle equatorial region but not for spindle microtubule assembly in female meiosis. (A) Subito localises to equatorial regions in wild type and the incenp^QA26 mutant. (B) Instability of the spindle equatorial region in the subito¹ mutant. An example of metaphase I-arrested subito oocytes expressing GFP--tubulin. An ectopic pole (arrowhead) was formed at the spindle equatorial region and merged with one of the main poles. (C) Time taken from nuclear envelope breakdown to the first appearance of spindle microtubules. The subito mutant did not show a significant difference from wild type (P=0.45; n=6). Scale bars: 10 µm.