Fig. 7. Molecular characterisation of Tshz3^lacZ/lacZ ureters. (A-F) In situ hybridisation on transverse sections of E15.5 proximal ureters of wild type (A,C,E) and Tshz3-null mutant (B,D,F). Patterns of expression (red dots) were similar in both genotypes, with Shh expressed in urothelia (A,B), and Ptch1 (C,D) and Bmp4 (E,F) expressed in peri-urothelial cells. Silver grains were pseudo-coloured in Adobe Photoshop. (G,H) E14.5 proximal ureter sections from wild type (G) or null mutant (H) immunostained for pSMAD 1/5/8 (red) and DAPI (blue).