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Figure 4


Fig. 4. SGPs form normally in mutants, but some germ cells fail to associate with SGPs. (A) Stage 13 wild-type embryo. PGCs form elongated clusters. (B) Stage 13 wild-type embryo. SGPs align with the PGCs. (C) Stage 13 out embryo. Arrows indicate errant germ cells that failed to align with SGPs. (D) Stage 13 out embryo. SGP formation appears normal. (E) Stage 11 p53 embryo. PGCs migrated toward the SGPs. (F) Stage 11 p53 embryo. SGPs were specified in three clusters in parasegments 10-12 (arrows). (G) Stage 13 p53 embryo. Arrows indicate errant germ cells that failed to align with SGPs. (H) Stage 13 p53 embryo. SGPs appear normal. Anterior is left in A-F, and downwards in G and H. A, C, E and G are stained with a Vasa antibody, and B, D, F, and H are stained with an EYA antibody.