Fig. 7. Molecular identification of out. (A) A P-element insertion in CG8062 disrupts germ cell PCD. This allele fails to complement other out mutants. The P-element lies between exons 1 and 2. (B) Amino acid sequence of OUT. Three out alleles introduce premature stop codons in the CG8062 transcript. The wild-type OUT peptide comprises 655 amino acids. The positions of the stop codons are indicated by the bold underlined letters. (C) Schematic of the predicted OUT protein. Positions of the out nonsense mutations are indicated by arrows. The introduced stop codons would result in truncated proteins of 224, 276 and 310 amino acids for out², out¹ and out⁵, respectively.