Fig. 6. The Xist locus on X^pA fails to establish the repressive histone modification. Chromatin of the visceral endoderm was used for ChIP assays with the antibodies against the respective histone modifications. The immunoprecipitated chromatin was quantified by real-time PCR using Xist1F and Xist101R as primers, which amplify the first 101 bp sequence of Xist exon 1. The value for each modification, which is shown as relative abundance of the chromatin immunoprecipitated by each antibody compared with the input, was obtained from two or three independent experiments. Values are means ± s.d.