Fig. 6. Co-localization of FILIA and MATER by confocal microscopy. Mouse oocytes, eggs, one-cell, two-cell and four-cell embryos, morulae and blastocysts (E3.5) were fixed, permeabilized and stained with peptide-purified antibodies to FILIA (green) and MATER (red). Co-localization of FILIA and MATER was determined by Merge (yellow) and oocytes/eggs/embryos were imaged by differential interference contrast (DIC) microscopy. Scale bar: 20 µm.